Search results for " Human red blood cell"

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Oxysterol mixture in hypercholesterolemia-relevant proportion causes oxidative stress-dependent eryptosis.

2014

Background/Aims: Oxysterol activity on the erythrocyte (RBC) programmed cell death (eryptosis) had not been studied yet. Effects of an oxysterol mixture in hyper-cholesterolemic-relevant proportion, and of individual compounds, were investigated on RBCs from healthy humans. Methods: Membrane phosphatidylserine (PS) externalization, calcium entry, ROS production, amino-phospholipid translocase (APLT) activity were evaluated by cytofluorimetric assays, cell volume from forward scatter. Prostaglandin PGE2 was measured by ELISA; GSH-adducts and lipoperoxides by spectrophotometry. Involvement of protein kinase C and caspase was investigated by inhibitors staurosporin, calphostin C, and Z-DEVD-FM…

ErythrocytesPhysiologyEryptosisApoptosisPharmacologylcsh:PhysiologyAntioxidantschemistry.chemical_compoundPhospholipid scramblingSettore BIO/10 - Biochimicapolycyclic compoundslcsh:QD415-436PhosphatidylserineKetocholesterolsProtein Kinase Clcsh:QP1-981OxysterolsPhosphatidylserineErythrocyteCalphostin CBiochemistryCaspaseslipids (amino acids peptides and proteins)AntioxidantReactive Oxygen SpecieHumanProgrammed cell deathOxysterolHypercholesterolemiachemistry.chemical_elementPhosphatidylserinesCalciumCalcium ChannelDinoprostonelcsh:BiochemistryOxysterolLipid oxidationHumansCalphostinHypercholesterolemia Human red blood cell Oxysterols Eryptosis Oxidative stressKetocholesterolApoptosiOxidative StreCaspaseOxidative StresschemistryCalciumCalcium ChannelsReactive Oxygen SpeciesEryptosiHuman red blood cellCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

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